ECM29 Antibody

Western Blot: ECM29 Antibody [NB100-74407] - Analysis of Ecm29 in HeLa cells.

Western Blot: ECM29 Antibody [NB100-74407] - Figure 1 shows a Western blot of Ecm29 in HeLa cells. Figure 2 shows immunofluorescent staining of Ecm29 in HeLa cells.

Product Discontinued

ECM29 Antibody Summary

• Immunogen: Synthetic peptide corresponding to the C-terminal residues R(1821) P E L Q E K A A L L K K T L E N L E(1839) of human Ecm29.
• Specificity: Ecm29
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIAA0368
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:200
• Application Notes: WB: Analysis of HeLa cell extracts. Detects an approx. 205 kDa protein representing Ecm29.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole serum
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for ECM29 Antibody

• ECM29 homolog, proteasome accessory protein
• ECM29
• FLJ22036
• homolog of yeast Ecm29
• KIAA0368
• KIAA1962
• proteasome-associated protein ECM29 homolog

Background

Ecm29 appears to be one of three proteins which associate significantly with proteasomes. Ecm29 has been shown to enhance the stability of the proteasome. Ecm29 deletion has been shown to produce 26S proteasomes which dissociated in the absence of ATP. It was thus suggested that Ecm29 tethers the regulatory complex to the proteasome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIAA0368
• Entrez:
  • 23392(Human)
  • 230249(Mouse)
• Uniprot:
  • Q5VYK3(Human)