ECM-1/Secretory Component P85 Antibody (SC05)

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) [NBP2-29464] - Secretory Component / ECM1 Antibody (SC05) [NBP2-29464] - Formalin-fixed, paraffin-embedded normal human intestine (20X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) [NBP2-29464] - Formalin-fixed, paraffin-embedded normal human intestine (10X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) [NBP2-29464] - Secretory Component / ECM1 Antibody (SC05) [NBP2-29464] - Staining of normal human small intestine. Note cytoplasmic and membrane staining.

Product Discontinued

ECM-1/Secretory Component P85 Antibody (SC05) Summary

• Immunogen: Partially purified ECM1 from human colostrum was used as immunogen to generate the antibody (Bartek, 1989).
• Localization: Cell surface and cytoplasmic
• Specificity: This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ECM1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug protein lysate
• Application Notes: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for ECM-1/Secretory Component P85 Antibody (SC05)

• ECM1
• extracellular matrix protein 1
• p85
• Secretory Component Glycoprotein
• Secretory Component P85

Background

Secretory component is an ~80 kDa glycoprotein important in mucosal immunity. Secretory component is the extracellular cleaved ectodomain of the polymeric immunoglobulin (Ig) receptor (pIgR) which transports Igs across mucosal membranes into secretions (reviewed in Johansen, 2011). IgA is the main immunoglobulin in secretions and pIgR is highly expressed in intestinal epithelial cells (ECs). Dimeric IgA (dIgA) manufactured by antibody producing plasma cells in the intestinal lamina propria binds to pIgR at the basolaterial membrane surface of ECs. pIgR-dIgA complexes are internalized and transcytosed across ECs to the apical surface. Secretory component is then proteolytically cleaved off from the pIgR and released into the lumen together with dIgA. Secreted IgA (SIgA) consists of dIgA + secretory component. SIgA protects the mucosa from microbial invasion, and bound Secretory component enhances immune function by inhibiting SIgA proteolytic degradation. The SC05 antibody clone reacts with both free and bound secretory component (Bartek, 1989); it does not react in western blots of cell lines that lack secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a useful marker for identifying subpopulations of epithelial cells and epithelial differentiation (Fichorova, 1997 & 1998). Secretory component antibodies have also been part of antibody marker panels for differential identification of glandular carcinomas (Brown, 1993; Harris, 1981; Kalhounou, 2010). The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression (Bartek, 1990; Johansen, 2011). Since the presence of secretory component implies immunological function, the antibody can help identify cells regulating immunological processes (Fichorova, 1999). The antibody can also be used to indirectly assess SIgA expression since SIgA contains bound secretory component (Vancikoa, 2003). The secretory component antibody SC05 clone is also known as clone COMPO2 (Tang, 1985) in the published literature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ECM-1/Secretory Component P85 Antibody (NBP2-29464)(7)

Publications using NBP2-29464

• Philley JV, Kannan A, Griffith DE et al. Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease. Oncotarget. 2017-03-14 [PMID: 28160560] (FLOW, Human)
• Polosukhin VV, Cates JM, Lawson WE et al. Bronchial secretory immunoglobulin a deficiency correlates with airway inflammation and progression of chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 2011-08-01 [PMID: 21512171] (IHC-P)
  
  Details:
  IHC (P): lung, see publications for details.
• Gologan A, Acquafondata M, Dhir R et al. Polymeric immunoglobulin receptor-negative tumors represent a more aggressive type of adenocarcinomas of distal esophagus and gastroesophageal junction. Arch Pathol Lab Med. 2008-08-01 [PMID: 18684029] (IHC-P)
  
  Details:
  IHC (P): gastric adenocarcinomas and intestinal metaplasia (Figs 1-4, Tables 1-3).
• Tang QJ, Tao KZ, Yun-Liu et al. Immunocytochemical localization of secretory component in Paneth cell secretory granules-rat Paneth cells participate in acquired immunity. J Mol Histol. 2005-06-01 [PMID: 16189640]
• Fichorova RN, Anderson DJ. Differential expression of immunobiological mediators by immortalized human cervical and vaginal epithelial cells. Biol Reprod. 1999-02-01 [PMID: 9916021] (IHC-Fr, ICC/IF)
  
  Details:
  IHC (F) and ICC: epithelial tissue and cell lines all originating from the vagina or cervix, Fig 3A-F.
• Fichorova RN, Rheinwald JG, Anderson DJ. Generation of papillomavirus-immortalized cell lines from normal human ectocervical, endocervical, and vaginal epithelium that maintain expression of tissue-specific differentiation proteins. Biol Reprod. 1997-10-01 [PMID: 9314589] (ICC, IHC-Fr)
  
  Details:
  IHC (F) and ICC: epithelial tissue and tumorigenic (HeLa), immortalized and primary cell lines all originating from the vagina or cervix, Figs 2, 3 & Table 2.
• Bartek J, Bartkova J, Taylor-Papadimitriou J. Keratin 19 expression in the adult and developing human mammary gland. Histochem J. 1990-10-01 [PMID: 1705251] (IHC-P, Human)
  
  Details:
  IHC (P): human breast, Fig 3C, D. Note: Tissues were fixed in methacarn and embedded in paraffin.

Review for ECM-1/Secretory Component P85 Antibody (NBP2-29464) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ilya Ulasov IHC-P Human 02/22/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Breast cancer tissue
• Species: Human

Bioinformatics

• Gene Symbol: ECM1
• Uniprot:
  • Q16610()