EBAG9/RCAS1 Antibody (4A10) - Azide and BSA Free

Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04] - Analysis of EBAG9 expression in HepG2.

Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04] - EBAG9 monoclonal antibody (M04), clone 4A10. Western Blot analysis of EBAG9 expression in MCF-7 ( Cat # L046V1 ).

Western Blot: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04] - Analysis of EBAG9 expression in transfected 293T cell line by EBAG9 monoclonal antibody (M04), clone 4A10. Lane 1: EBAG9 transfected lysate (Predicted MW: 23.54 KDa). Lane 2: Non-transfected lysate.

Sandwich ELISA: EBAG9/RCAS1 Antibody (4A10) [H00009166-M04] - Detection limit for recombinant GST tagged EBAG9 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 4A10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

EBAG9/RCAS1 Antibody (4A10) - Azide and BSA Free Summary

• Immunogen: EBAG9 (AAH17729, 1 a.a. ~ 213 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAITQFRLFKFCTCLATVFSFLKRLICRSGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGNGNVATQQNSLEQLEPDYFKDMTPTIRKTQKIVIKKREPLNFGIPDGSTGFSSRLAATQDLPFIHQSSELGDLDTWQENTNAWEEEEDAAWQAEEVLRQQKLADREKRAAEQQRKKMEKEAQRLMKKEQNKIGVKLS
• Specificity: EBAG9 - estrogen receptor binding site associated, antigen, 9 (4A10)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EBAG9
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EBAG9/RCAS1 Antibody (4A10) - Azide and BSA Free

• Cancer-associated surface antigen RCAS1
• EB9cancer associated surface antigen
• estrogen receptor binding site associated, antigen, 9
• Estrogen receptor-binding fragment-associated gene 9 protein
• RCAS1PDAF
• receptor-binding cancer antigen expressed on SiSo cells

Background

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EBAG9
• Entrez:
  • 9166(Human)
• Uniprot:
  • AAH17729(Human)