E2F8 Antibody - BSA Free

Western Blot: E2F8 Antibody [NBP1-52650] - Detection of human E2F8 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F8 antibody NBP1-52650 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: E2F8 Antibody [NBP1-52650] - Detection of human E2F8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F8 antibody NBP1-52650(lot NBP1-52650-3) used for IP at 6 ug per reaction. E2F8 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-52650-2). For blotting immunoprecipitated E2F8, NBP1-52650 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

E2F8 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit E2F8 Antibody - BSA Free (NBP1-52650) is a polyclonal antibody validated for use in WB and IP. Anti-E2F8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 817 and 867 of human E2F Transcription Factor 8 using the numbering given in entry NP_078956.2 (GeneID 79733).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: E2F8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for E2F8 Antibody - BSA Free

• E2F family member 8
• E2F transcription factor 8
• E2F-8
• FLJ23311
• transcription factor E2F8

Background

E2F transcription factors, such as E2F8, are essential for orchestrating expression of genes required for cell cycle progression and proliferation (Christensen et al., 2005 [PubMed 16179649]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E2F8 Antibody (NBP1-52650)(1)

Publication using NBP1-52650

• Leslie PL, Chao YL, Tsai YH et al. Histone deacetylase 11 inhibition promotes breast cancer metastasis from lymph nodes Nat Commun 2019-09-13 [PMID: 31519896] (WB, Human)

Bioinformatics

• Gene Symbol: E2F8