Dystroglycan [p Tyr893] Antibody (Poly6171)

Product Discontinued

Dystroglycan [p Tyr893] Antibody (Poly6171) Summary

• Immunogen: Modified peptide
• Modification: p Tyr893
• Specificity: Binds to utrophin when unphosphorylated, c-Src, Fyn, Csk, NCK, and SHC when phosphorylated. Binds to WW domain in dystrophin
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DAG1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Human, reacts with Tyr893-phosphorylated dystroglycan.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Notes

Distribution: Beta subunit integral membrane protein, Alpha subunit extracellular; ubiquitously expressed. Muscle and non-muscle isoforms differ by carbohydrate moieties (not protein sequence)
Function: Adhesion molecule responsible for interactions between extracellular matrix and subsarcolemmal cytoskeleton. Binds to lamin in the matrix and dystrophin in the cytoskeleton
Regulation: Phosphorylation regulates the association of interacting proteins
Interaction: Binds to utrophin when unphosphorylated, c-Src, Fyn, Csk, NCK, and SHC when phosphorylated. Binds to WW domain in dystrophin

Alternate Names for Dystroglycan [p Tyr893] Antibody (Poly6171)

• A3a
• AGRNR
• DAG1
• Dag-1
• DAG156DAG
• dystroglycan 1 (dystrophin-associated glycoprotein 1)
• Dystroglycan
• Dystrophin-associated glycoprotein 1

Background

Dystroglycan (also known as dystrophin-associated glycoprotein 1) is a member of the dystroglycan family that contains the WWW binding motif PPxY. This protein has alpha and Beta subunits with approximate molecular weights of 156 kD and 43-50 kD, respectively. The dystroglycan Beta subunit is an integral membrane protein, the a subunit is alpha ubiquitously expressed extracellular protein. Muscle and non-muscle isoforms differ by carbohydrate moieties (not protein sequence). Dystroglycan functions as an adhesion molecule responsible for interactions between extracellular matrix and the subsarcolemmal cytoskeleton. Dsytroglycan binds to lamin in the matrix and dystrophin in the cytoskeleton; phosphorylation regulates the association of interacting proteins. Dystroglycan binds to utrophin when unphosphorylated; c-Src, Fyn, Csk, NCK, and SHC when phosphorylated. Dystroglycan interacts with dystrophin through the WW domain. The Poly6171 antibody recognizes human phosphorylated dystroglycan (Tyr893) and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DAG1
• Entrez:
  • 1605(Human)