DUSP4 Antibody

Images

 
Western Blot: DUSP4 Antibody [H00001846-D01P] - Analysis of DUSP4 expression in transfected 293T cell line by DUSP4 polyclonal antibody.Lane 1: DUSP4 transfected lysate(43.00 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: DUSP4 Antibody [H00001846-D01P] - Analysis of antibody to DUSP4 on HeLa cell. Antibody concentration 10 ug/ml.
Proximity Ligation Assay: DUSP4 Antibody [H00001846-D01P] - Analysis of protein-protein interactions between DUSP4 and MAPK9. HeLa cells were stained with anti-DUSP4 rabbit purified polyclonal 1:1200 and anti-MAPK9 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DUSP4 Antibody Summary

Immunogen
DUSP4 (NP_001385.1, 1 a.a. - 394 a.a.) full-length human protein. MVTMEELREMDCSVLKRLMNRDENGGGAGGSGSHGTLGLPSGGKCLLLDCRPFLAHSAGYILGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSATEPLDLGCSSCGTPLHDQGGPVEILPFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDNHKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCAAEAASPSGPLRERGKTPATPTSQFVFSFPVSVGVHSAPSSLPYLHSPITTSPSC
Specificity
DUSP4 - dual specificity phosphatase 4,
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DUSP4
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Proximity Ligation Assay
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DUSP4 Antibody

  • dual specificity phosphatase 4
  • Dual specificity protein phosphatase hVH2
  • EC 3.1.3.16
  • EC 3.1.3.48
  • HVH2serine/threonine specific protein phosphatase
  • MAP kinase phosphatase 2
  • Mitogen-activated protein kinase phosphatase 2
  • MKP-2dual specificity protein phosphatase 4
  • MKP2VH1 homologous phosphatase 2
  • TYP
  • VH2

Background

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DUSP4
Entrez
Uniprot