DUSP2 Antibody

Images

 
Western Blot: DUSP2 Antibody [NBP2-14934] - analysis of DUSP2 in HeLa cell lysate using anti-DUSP2 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: DUSP2 Antibody [NBP2-14934] - Paraformaldehyde-fixed HeLa, using DUSP2/PAC1 antibody at 1:1000 dilution.
Immunohistochemistry-Paraffin: DUSP2 Antibody [NBP2-14934] - Paraffin-embedded Oral CA, using antibody at 1:100 dilution.
Western Blot: DUSP2 Antibody [NBP2-14934] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE DUSP2(PAC-1 ) antibody, antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DUSP2 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DUSP2/PAC1. The exact sequence is proprietary.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DUSP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-14934 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DUSP2 Antibody

  • dual specificity protein phosphatase 2
  • PAC-1
  • dual specificity phosphatase 2
  • PAC1

Background

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1 and ERK2, is predominantly expressed in hematopoietic tissues, and is localized in the nucleus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DUSP2 Antibody (NBP2-14934) (0)

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Review for DUSP2 Antibody (NBP2-14934) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-14934:
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WB
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Images Ratings Applications Species Date Details
Western Blot DUSP2 NBP2-14934
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reviewed by:
Anonymous
WB Human 10/14/2014
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Summary

ApplicationWestern Blot
Sample TestedCell lysate from HeLa cells
SpeciesHuman

Blocking

Blocking Details5% BSA in 1X PBS

Primary Anitbody

Dilution Ratio1:1000 in 1X PBS with troton X-100 (0.1%) at 4 degree O/N

Secondary Antibody

Secondary DescriptionGoat Anti-Rabbit IgG - HRP (Life Tech)
Secondary Manufacturer Cat#65-6120
Secondary Concentration1:20K

Details

Detection NotesECL, 20 seconds exposure with film

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DUSP2 Antibody (NBP2-14934) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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2
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1
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Anonymous
10/14/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol DUSP2
Uniprot