DUSP10 Antibody

Western Blot: DUSP10 Antibody [NB100-888] - Human Liver lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DUSP10 Antibody Summary

• Description: Novus Biologicals Goat DUSP10 Antibody (NB100-888) is a polyclonal antibody validated for use in WB, ELISA and IP. Anti-DUSP10 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CRILTPKLMGVETVV corresponding to C-Terminus according to NP_009138.1, NP_653329.1, NP_653330.1.
• Specificity: This antibody is expected to recognise both reported Human isofoms. The variants NP_653329.1 and NP_653330.1 represent identical protein.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DUSP10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 53 kDa band observed in human liver lysate. Use in immunoprecipitation reported in scientific literature (PMID: 16166642).
• Control:

  DUSP10 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DUSP10 Antibody

• dual specificity phosphatase 10
• dual specificity phosphatase MKP-5
• EC 3.1.3.16
• EC 3.1.3.48
• MAP kinase phosphatase 5
• Mitogen-activated protein kinase phosphatase 5
• MKP-5dual specificity protein phosphatase 10
• MKP5serine/threonine specific protein phosphatase

Background

DUSP10 is involved in the inactivation of MAP kinases, having a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DUSP10 Antibody (NB100-888)(2)

Publications using NB100-888

• Kadoya T, Khurana A, Tcherpakov M et al. JAMP, a Jun N-terminal kinase 1 (JNK1)-associated membrane protein, regulates duration of JNK activity. Mol Cell Biol. 2005-10-01 [PMID: 16166642] (IP)
• Tanoue T, Moriguchi T, Nishida E. Molecular cloning and characterization of a novel dual specificity phosphatase, MKP-5. J Biol Chem 1999-07-09 [PMID: 10391943]

Bioinformatics

• Gene Symbol: DUSP10
• Entrez:
  • 11221(Human)
• Uniprot:
  • NP_009138(Human)