Drosha Antibody (JU33-01)

Western Blot: Drosha Antibody (JU33-01) [NBP2-75475] - Analysis of Drosha on K562 cell lysates using anti-Drosha antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Drosha Antibody (JU33-01) [NBP2-75475] - Staining Drosha in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Drosha Antibody (JU33-01) [NBP2-75475] - Analysis of paraffin-embedded human placenta tissue using anti-Drosha antibody. Counter stained with hematoxylin.

Flow Cytometry: Drosha Antibody (JU33-01) [NBP2-75475] - Analysis of Jurkat cells with Drosha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Western Blot: Drosha Antibody (JU33-01) [NBP2-75475] - Analysis of Drosha on Hela cell and SiHa cell lysates using anti-Drosha antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Drosha Antibody (JU33-01) [NBP2-75475] - Staining Drosha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Drosha Antibody (JU33-01) [NBP2-75475] - Staining Drosha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Drosha Antibody (JU33-01) [NBP2-75475] - Analysis of paraffin-embedded human kidney tissue using anti-Drosha antibody. Counter stained with hematoxylin.

Product Discontinued

Drosha Antibody (JU33-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Drosha aa 30-79 / 1,374. (SwissProt: Q9NRR4 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DROSHA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 159 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Drosha Antibody (JU33-01)

• drosha, double-stranded RNA-specific endoribonuclease
• drosha, ribonuclease type III
• EC 3.1.26.3
• ETOHI2
• nuclear RNase III Drosha
• p241
• Protein Drosha
• putative protein p241 which interacts with transcription factor Sp1
• putative ribonuclease III
• RANSE3L
• ribonuclease 3
• Ribonuclease III
• ribonuclease III, nuclear
• RN3nuclear
• RNase III

Background

Members of the ribonuclease III superfamily of double-stranded (ds) RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells (Fortin et al., 2002 [PubMed 12191433]). The RNase III Drosha is the core nuclease that executes the initiation step of microRNA (miRNA) processing in the nucleus (Lee et al., 2003 [PubMed 14508493]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DROSHA