DPM3 Antibody

Western Blot: DPM3 Antibody [NBP2-26105] - Staining of K562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DPM3 Antibody Summary

• Description: Novus Biologicals Goat DPM3 Antibody (NBP2-26105) is a polyclonal antibody validated for use in WB and ELISA. Anti-DPM3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence HDCEDAARELQSQ corresponding to internal region according to NP_061846.2, NP_714963.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_061846.2 and NP_714963.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DPM3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 10+13 kDa band bands observed in K562 lysate (calculated MW of 13.3 kDa band according to NP_061846.2 and 10.1 kDa band according to NP_714963.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DPM3 Antibody

• CDG1O
• Dolichol-phosphate mannose synthase subunit 3
• dolichol-phosphate mannosyltransferase subunit 3
• Dolichyl-phosphate beta-D-mannosyltransferase subunit 3
• dolichyl-phosphate mannosyltransferase polypeptide 3
• DPM synthase complex subunit 3
• Mannose-P-dolichol synthase subunit 3
• MGC125904
• MGC125905
• MGC34275
• MPD synthase subunit 3
• prostin 1
• Prostin-1

Background

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a subunit of dolichyl-phosphate mannosyltransferase and acts as a stabilizer subunit of the dolichyl-phosphate mannosyltransferase complex. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DPM3 Antibody (NBP2-26105)(1)

Publication using NBP2-26105

• Manos EJ, Kim ML, Kassis J et al. Dolichol-phosphate-mannose-3 (DPM3)/prostin-1 is a novel phospholipase C-gamma regulated gene negatively associated with prostate tumor invasion Oncogene 2001-05-17 [PMID: 11420690]

Bioinformatics

• Gene Symbol: DPM3
• Entrez:
  • 54344(Rat)
  • 54344(Rat)