DPH2 Antibody (5B10) - Azide and BSA Free

Western Blot: DPH2 Antibody (5B10) [H00001802-M02] - DPH2 monoclonal antibody (M02), clone 5B10. Analysis of DPH2 expression in PC-12.

Immunocytochemistry/Immunofluorescence: DPH2 Antibody (5B10) [H00001802-M02] - Analysis of monoclonal antibody to DPH2 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: DPH2 Antibody (5B10) [H00001802-M02] - DPH2 monoclonal antibody (M02), clone 5B10 Analysis of DPH2 expression in HeLa.

Sandwich ELISA: DPH2 Antibody (5B10) [H00001802-M02] - Detection limit for recombinant GST tagged DPH2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 5B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DPH2 Antibody (5B10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DPH2 (NP_001375, 125 a.a. ~ 235 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RQRSVALELCVKAFEAQNPDPKAPVVLLSEPACAHALEALATLLRPRYLDLLVSSPAFPQPVGSLSPEPMPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLS
• Specificity: DPH2 - DPH2 homolog (S. cerevisiae)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DPH2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DPH2 Antibody (5B10) - Azide and BSA Free

• Diphthamide biosynthesis protein 2 homolog-like 2
• diphthamide biosynthesis protein 2
• diptheria toxin resistance protein required for diphthamide biosynthesis-like 2
• diptheria toxin resistance protein required for diphthamide biosynthesis-like 2(S. cerevisiae)
• DPH2 homolog (S. cerevisiae)
• DPH2 homolog
• DPH2L2diphthamide biosynthesis-like protein 2
• DPH2-like 2 (S. cerevisiae)
• DPH2-like 2
• hsDph2

Background

This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPH2
• Entrez:
  • 1802(Human)
• OMIM:
  • 603456(Human)
• Uniprot:
  • NP_001375(Human)