DPH2 Antibody (3F7)

Images

 
Western Blot: DPH2 Antibody (3F7) [H00001802-M07] - Analysis of DPH2 expression in transfected 293T cell line by DPH2 monoclonal antibody (M07), clone 3F7. Lane 1: DPH2 transfected lysate (Predicted MW: 52.1 KDa). Lane ...read more
Immunocytochemistry/ Immunofluorescence: DPH2 Antibody (3F7) [H00001802-M07] - Analysis of monoclonal antibody to DPH2 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: DPH2 Antibody (3F7) [H00001802-M07] - Detection limit for recombinant GST tagged DPH2 is 3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
3F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

DPH2 Antibody (3F7) Summary

Immunogen
DPH2 (AAH16956, 1 a.a. - 489 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MESMFSSPAEAALQRETGVPGLLTPLPDLDGVYELERVAGFVRDLGCERVALQFPDQLLGDAVAVAARLEETTGSKMFILGDTAYGSCCVDVLGAEQAGAQALIHFGPACLSPPARPLPVAFVLRQRSVALELCVKAFEAQNPDPKAPVVLLSEPACAHALEALATLLRPRYLDLLVSSPAFPQPVGSLSPEPMPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRLLLGWAPGQPFSSCCPDTGKTQDEGARAGRLRARRRYLVERARDARVVGLLAGTLGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQLSGSFFQPILAPCELEAACNPAWPPPGLAPHLTHYADLLPGSPFHVALPPPESELWETPDVSLITGDLRPPPAWKSSNDHGSLALTPRPQLELAESSPAASFLSSRSWQGLEPRLGQTPVTEAVSGRRGIAIAYEDEGSG
Specificity
DPH2 - DPH2 homolog (S. cerevisiae) (3F7)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DPH2
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DPH2 Antibody (3F7)

  • Diphthamide biosynthesis protein 2 homolog-like 2
  • diphthamide biosynthesis protein 2
  • diptheria toxin resistance protein required for diphthamide biosynthesis-like 2
  • diptheria toxin resistance protein required for diphthamide biosynthesis-like 2(S. cerevisiae)
  • DPH2 homolog (S. cerevisiae)
  • DPH2 homolog
  • DPH2L2diphthamide biosynthesis-like protein 2
  • DPH2-like 2 (S. cerevisiae)
  • DPH2-like 2
  • hsDph2

Background

This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DPH2
Entrez
Uniprot