DORFIN Antibody

Product Discontinued

DORFIN Antibody Summary

• Immunogen: Peptide with sequence C-QTMELKVAIQTEI corresponding to C-Terminus according to NP_056250.3, NP_904355.1.
• Specificity: Both variants (NP_056250.3; NP_904355.1) encode the same protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RNF19A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: No signal obtained yet but low background observed in human skeletal muscle, human kidney, human liver, human heart and human brain lysates at up to 2ug/ml.
• Control:

  DORFIN Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for DORFIN Antibody

• DKFZp566B1346
• dorfin
• Double ring-finger protein
• E3 ubiquitin-protein ligase RNF19A
• EC 6.3.2
• EC 6.3.2.-
• p38
• protein p38 interacting with transcription factor Sp1
• RING finger protein 19 isoform
• ring finger protein 19
• ring finger protein 19Ap38 protein
• ring-IBR-ring domain containing protein Dorfin
• RNF19

Background

Dorfin is an E3 ubiquitin ligase which mediates ubiquitination of cellular proteins. Dorfin contains two RING-finger motifs and an IBR (in between RING fingers) motif. Dorfin interacts with UBE2L3/UBCH7 and UBE2E2/UBCH8, but not other ubiquitin-conjugating enzymes. Dorfin is of interest in Parkinson's Disease, as it binds and ubiquitylates synphilin 1 (SNCAIP - interacts with alpha synuclein) in neurons. Furthermore, Dorfin expression is increased in Lewy bodies, the characteristic neuronal inclusions in Parkinson's diseased brains. In normal subjects, Dorfin is widely expressed at low levels with higher levels found in the heart and is ubiquitously expressed in the central nervous system. Alternatively spliced transcript variants encoding the same protein have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DORFIN Antibody (NB300-873)(1)

Publication using NB300-873

• Niwa J, Ishigaki S, Doyu M et al. A novel centrosomal ring-finger protein, dorfin, mediates ubiquitin ligase activity. Biochem Biophys Res Commun 2001-03-02 [PMID: 11237715]

Bioinformatics

• Gene Symbol: RNF19A
• Entrez:
  • 25897(Human)
• Uniprot:
  • NP_056250.(Human)