DORFIN Overexpression Lysate

Western Blot: DORFIN Overexpression Lysate (Adult Normal) [NBL1-15441] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DORFIN.

• Reactivity: Hu
• Applications: WB

DORFIN Overexpression Lysate Summary

• Description: Transient overexpression lysate of ring finger protein 19A (RNF19A), transcript variant 2 The lysate was created in HEK293T cells, using Plasmid ID RC222087 and based on accession number NM_015435. The protein contains a C-MYC/DDK Tag.
• Gene: RNF19A

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 90.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DORFIN Overexpression Lysate

• DKFZp566B1346
• dorfin
• Double ring-finger protein
• E3 ubiquitin-protein ligase RNF19A
• EC 6.3.2
• EC 6.3.2.-
• p38
• protein p38 interacting with transcription factor Sp1
• RING finger protein 19 isoform
• ring finger protein 19
• ring finger protein 19Ap38 protein
• ring-IBR-ring domain containing protein Dorfin
• RNF19

Background

The protein encoded this gene contains two RING-finger motifs and an IBR (in between RING fingers) motif. This protein is an E3 ubiquintin ligase that is localized in Lewy bodies (LBs), a characteristic neuronal inclusion in Parkinson's disease (PD) brains. This protein interacts with UBE2L3/UBCH7 and UBE2E2/UBCH8, but not other ubiquitin-conjugating enzymes. This protein is found to bind and ubiquitylate synphilin 1 (SNCAIP), which is a interacting protein of alpha synuclein in neurons, and a major component of LB. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: RNF19A