Recombinant Human Dopa Decarboxylase/DDC Protein

Product Discontinued

Recombinant Human Dopa Decarboxylase/DDC Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to amino acids 1 to 480 of Human Dopa Decarboxylase/DDC
  
  Source: E.coli
  
  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH TRSMNASEFR RRGKEMVDYV ANYMEGIEGR QVYPDVEPGY LRPLIPAAAP QEPDTFEDII NDVEKIIMPG VTHWHSPYFF AYFPTASSYP AMLADMLCGA IGCIGFSWAA SPACTELETV MMDWLGKMLE LPKAFLNEKA GEGGGVIQGS ASEATLVALL AARTKVIHRL QAASPELTQA AIMEKLVAYS SDQAHSSVER AGLIGGVKLK AIPSDGNFAM RASALQEALE RDKAAGLIPF FMVATLGTTT CCSFDNLLEV GPICNKEDIW LHVDAAYAGS AFICPEFRHL LNGVEFADSF NFNPHKWLLV NFDCSAMWVK KRTDLTGAFR LDPTYLKHSH QDSGLITDYR HWQIPLGRRF RSLKMWFVFR MYGVKGLQAY IRKHVQLSHE FESLVRQDPR FEICVEVILG LVCFRLKGSN KVNEALLQRI NSAKKIHLVP CHLRDKFVLR FAICSRTVES AHVQRAWEHI KELAADVLRA ERE
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DDC
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 56.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human Dopa Decarboxylase/DDC Protein

• AADC
• AADCaromatic-L-amino-acid decarboxylase
• DDC
• dopa decarboxylase (aromatic L-amino acid decarboxylase)
• Dopa Decarboxylase
• EC 4.1.1
• EC 4.1.1.28

Background

Dopa decarboxylase, also known as DDC, is a homodimeric, pyridoxal phosphate dependent enzyme. Dopa decarboxylase is a protein implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters: dopamine and serotonin which both play key roles in many clinical disorders, including Parkinson's disease. Dopa decarboxylase is found in different areas of the brain and is particularly abundant in basal ganglia. Recombinant human Dopa decarboxylase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Dopa Decarboxylase/DDC Protein (NBC1-25854)(1)

Publication using NBC1-25854

• Ren L, Chen M, Hultborn H et al. Heterogenic distribution of aromatic L-amino acid decarboxylase neurons in the rat spinal cord Frontiers in Integrative Neuroscience. 2017-11-08 [PMID: 29225571] (WB, Human)

Bioinformatics

• Gene Symbol: DDC