DOK3 Antibody

Western Blot: DOK3 Antibody [NB100-838] - (2ug/ml) staining of Human Bone Marrow (A) and PBL (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: DOK3 Antibody [NB100-838] - (0.1ug/ml) of Human PBMC lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DOK3 Antibody Summary

• Immunogen: Peptide with sequence TRERRKGPAPCDRP corresponding to C-Terminus according to NP_079148.2.
• Localization: Cytoplasmic
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DOK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 53.3 kDa band according to NP_079148.2).
• Control:

  Blood Leukocyte Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DOK3 Antibody

• docking protein 3
• DOK3
• DOKL
• Dok-like protein
• Downstream of tyrosine kinase 3
• FLJ22570
• FLJ39939

Background

This protein is involved in insulin receptor binding activity and RAS protein signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DOK3 Antibody (NB100-838)(1)

Publication using NB100-838

• Lemay S, Davidson D, Latour S, Veillette A. Dok-3, a novel adapter molecule involved in the negative regulation of immunoreceptor signaling. Mol Cell Biol 2000-04-01 [PMID: 10733577]

Bioinformatics

• Gene Symbol: DOK3
• Entrez:
  • 79930(Human)
  • 27261(Mouse)
• Uniprot:
  • NP_005438.(Human)