DOK3 Antibody (642316)

Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 0.25 µg/mL of Mouse Anti-Human DOK3 Monoclonal Antibody (Catalog # MAB4795) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DOK3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu
• Applications: WB
• Clone: 642316
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

DOK3 Antibody (642316) Summary

• Immunogen: E. coli-derived recombinant human DOK3
  Gly181-Lys271
  Accession # Q7L591
• Specificity: Detects human DOK3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DOK3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DOK3 Antibody (642316)

• docking protein 3
• DOK3
• DOKL
• Dok-like protein
• Downstream of tyrosine kinase 3
• FLJ22570
• FLJ39939

Background

DOK3 (downstream of kinase 3) is a 53 kDa (predicted) member of the DOK family of cytoplasmic adaptor proteins and is expressed in B cells and macrophages.  Human DOK3 cDNA encodes 496 amino acids (aa) including one pleckstrin homology region (aa 63‑179) and an IRS-type phosphotyrosine binding domain (PTB) (aa 213‑317). Alternate splicing generates 330, 228 and 216 aa isoforms with N-terminal and C-terminal truncations and substitutions. Human DOK3 shares approximately 80% aa sequence identity with mouse and rat DOK3 within aa 181‑271, which are common to all four isoforms. Phosphorylation of tyrosine residues in DOK3 regulates its interaction with SH2 and SH3 containing proteins, leading to inhibition of signaling from immunoreceptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DOK3
• Uniprot:
  • Q7L591(Human)