DOK2 Antibody (OTI1A4)

Western Blot: DOK2 Antibody (1A4) [NBP2-00948] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK2.

Immunocytochemistry/Immunofluorescence: DOK2 Antibody (1A4) [NBP2-00948] Staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK2.

Flow Cytometry: DOK2 Antibody (1A4) [NBP2-00948] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DOK2 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clone: OTI1A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

DOK2 Antibody (OTI1A4) Summary

• Immunogen: Full length human recombinant protein of human DOK2 (NP_003965) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DOK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 45.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DOK2 Antibody (OTI1A4)

• docking protein 2
• docking protein 2, 56kD
• docking protein 2, 56kDa
• Dok-2
• Downstream of tyrosine kinase 2
• p56(dok-2)
• p56DOK
• p56dok-2

Background

The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DOK2
• Entrez:
  • 9046(Human)