![Western Blot: DOG1/TMEM16A Antibody [NBP1-60076] - Human Liver Antibody Dilution: 0.2 ug/ml.](http://images.novusbio.com/fullsize/DOG1-TMEM16A-Antibody-Western-Blot-NBP1-60076-img0007.jpg "Western Blot: DOG1/TMEM16A Antibody [NBP1-60076] - Human Liver Antibody Dilution: 0.2 ug/ml.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 0.5 mg/ml |
| Description | The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution. |
| Immunogen | Synthetic peptides corresponding to DOG1/TMEM16A (transmembrane protein 16A) The peptide sequence was selected from the middle region of DOG1/TMEM16A (NP_060513). Peptide sequence HGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSENKY. The peptide sequence for this immunogen was taken from within the described region. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ANO1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 111 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 2% Sucrose |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Affinity purified |
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| Publication using NBP1-60076 | Applications | Species |
|---|---|---|
| Li RS, Wang Y, Chen HS et al. TMEM16A contributes to angiotensin II-induced cerebral vasoconstriction via the RhoA/ROCK signaling pathway. Mol Med Rep 2016-04-01 [PMID: 26955761] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: DOG1/TMEM16A Antibody [NBP1-60076] - Tissue:Human Fetal Muscle. Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide](http://images.novusbio.com/fullsize/DOG1-TMEM16A-Antibody-Western-Blot-NBP1-60076-img0003.jpg "Western Blot: DOG1/TMEM16A Antibody [NBP1-60076] - Tissue:Human Fetal Muscle. Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide")
![Western Blot: DOG1/TMEM16A Antibody [NBP1-60076] - Human Muscle lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/DOG1-TMEM16A-Antibody-Western-Blot-NBP1-60076-img0002.jpg "Western Blot: DOG1/TMEM16A Antibody [NBP1-60076] - Human Muscle lysate, concentration 0.2-1 ug/ml.")
![Western Blot: DOG1/TMEM16A Antibody [NBP1-60076] - Sample Tissue: Human Fetal Muscle, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane](http://images.novusbio.com/fullsize/DOG1-TMEM16A-Antibody-Western-Blot-NBP1-60076-img0005.jpg "Western Blot: DOG1/TMEM16A Antibody [NBP1-60076] - Sample Tissue: Human Fetal Muscle, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane")
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