DNAJB6 Overexpression Lysate

Images

 
Western Blot: DNAJB6 Overexpression Lysate (Adult Normal) [NBL1-09940] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DNAJB6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DNAJB6 Overexpression Lysate Summary

Description
Transient overexpression lysate of DnaJ (Hsp40) homolog, subfamily B, member 6 (DNAJB6), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC200206 and based on accession number NM_058246. The protein contains a C-MYC/DDK Tag.
Gene
DNAJB6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
35.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DNAJB6 Overexpression Lysate

  • DJ4
  • DKFZp566D0824
  • DnaJ (Hsp40) homolog, subfamily B, member 6
  • dnaJ homolog subfamily B member 6
  • DnaJ
  • DnaJ-like 2 protein
  • FLJ42837
  • Heat shock protein J2
  • HHDJ1
  • HSJ2
  • HSJ-2
  • MGC1152
  • MRJMGC117297
  • MSJ1
  • MSJ-1

Background

This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript variant and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-67817
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-38988
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-77397
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IB, ICC/IF, IHC, IHC-P, KD
AF3298
Species: Hu, Mu
Applications: WB
NB120-2788
Species: Hu, Mu, Rt, Bv, Fe, Fi, Ha, Pm
Applications: WB, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N
NBP1-88019
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP1-06274
Species: Hu, Mu, Rt, Ce, Ch, Dr, Sh
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IHC-FrFl
NBP1-33387
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF1543
Species: Hu
Applications: WB, IHC
NBP1-83703
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-97512
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-92677
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NB100-74635
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP2-30949
Species: Hu
Applications: IHC, IHC-P
NBP1-69571
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-81293
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP1-77685
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-90824
Species: Hu
Applications: WB, ICC/IF
NBP2-02996
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBL1-09940
Species: Hu
Applications: WB

Publications for DNAJB6 Lysate (NBL1-09940) (0)

There are no publications for DNAJB6 Lysate (NBL1-09940).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DNAJB6 Lysate (NBL1-09940) (0)

There are no reviews for DNAJB6 Lysate (NBL1-09940). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DNAJB6 Lysate (NBL1-09940) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-09940

Additional DNAJB6 Products

Bioinformatics Tool for DNAJB6 Lysate (NBL1-09940)

Discover related pathways, diseases and genes to DNAJB6 Lysate (NBL1-09940). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DNAJB6 Lysate (NBL1-09940)

Discover more about diseases related to DNAJB6 Lysate (NBL1-09940).
 

Pathways for DNAJB6 Lysate (NBL1-09940)

View related products by pathway.

PTMs for DNAJB6 Lysate (NBL1-09940)

Learn more about PTMs related to DNAJB6 Lysate (NBL1-09940).

Blogs on DNAJB6

There are no specific blogs for DNAJB6, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DNAJB6 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol DNAJB6