DNAJB6 Overexpression Lysate

Western Blot: DNAJB6 Overexpression Lysate (Adult Normal) [NBL1-09940] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DNAJB6.

• Reactivity: Hu
• Applications: WB

DNAJB6 Overexpression Lysate Summary

• Description: Transient overexpression lysate of DnaJ (Hsp40) homolog, subfamily B, member 6 (DNAJB6), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC200206 and based on accession number NM_058246. The protein contains a C-MYC/DDK Tag.
• Gene: DNAJB6

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 35.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DNAJB6 Overexpression Lysate

• DJ4
• DKFZp566D0824
• DnaJ (Hsp40) homolog, subfamily B, member 6
• dnaJ homolog subfamily B member 6
• DnaJ
• DnaJ-like 2 protein
• FLJ42837
• Heat shock protein J2
• HHDJ1
• HSJ2
• HSJ-2
• MGC1152
• MRJMGC117297
• MSJ1
• MSJ-1

Background

This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript variant and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: DNAJB6