DNA Polymerase lambda Antibody

Western Blot: DNA Polymerase lambda Antibody [NB100-1358] - Staining of A549 (A) and HepG2 (B) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DNA Polymerase lambda Antibody Summary

• Immunogen: Peptide with sequence C-LGLPYREPAERDW corresponding to C-Terminus according to NP_037406.1.
• Localization: Nuclear
• Specificity: This product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core), but considering only the peptide that we used as immunogen, the two share the sequence YREP**R** at the C-terminus. Furthermore, the protein structures appear to be very similar in this region.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: POLL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5-1 ug/ml
• Application Notes: WB: Approx 60 kDa band observed in cell lysates of A549 and approx 60-70 kDa in cell lysates of HepG2 (calculated MW of 63.5k Da according to Human NP_037406.1).
• Control:

  DNA Polymerase lambda Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DNA Polymerase lambda Antibody

• BETAN
• DNA polymerase beta-2
• DNA polymerase beta-N
• DNA polymerase kappa
• DNA polymerase lambda
• EC 2.7.7.7
• EC 4.2.99.-
• FLJ46002
• Pol beta2
• Pol Lambda
• POLKAPPA
• polymerase (DNA directed), lambda

Background

GenBank Accession Number - NP_037406 DNA polymerase lambda (Pol lambda) is a member of the Pol X family that has properties in common with several other DNA polymerases. The specific role of Pol lambda has been speculated to be in non-homologous end-joining including DNA strand-breaks resulting from DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA Polymerase lambda Antibody (NB100-1358)(1)

Publication using NB100-1358

• Garcia-Diaz M, Bebenek K, Sabariegos R et al. DNA polymerase lambda, a novel DNA repair enzyme in human cells. J Biol Chem 2002-04-12 [PMID: 11821417]

Bioinformatics

• Gene Symbol: POLL
• Entrez:
  • 27343(Human)
  • 56626(Mouse)
• Uniprot:
  • NP_037406.(Human)