DNA Ligase IV Antibody (JM64-32)

Western Blot: DNA Ligase IV Antibody (JM64-32) [NBP2-75474] - Analysis of DNA Ligase IV on Hela cell using anti-DNA Ligase IV antibody at 1/1,000 dilution.Positive control:Lane 1: HepG2 Lane 2: HelaLane 3: Jurkat

Immunohistochemistry-Paraffin: DNA Ligase IV Antibody (JM64-32) [NBP2-75474] - Analysis of paraffin-embedded human placenta tissue using anti-DNA Ligase IV antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: DNA Ligase IV Antibody (JM64-32) [NBP2-75474] - Analysis of paraffin-embedded human colon cancer tissue using anti-DNA Ligase IV antibody. Counter stained with hematoxylin.

Product Discontinued

DNA Ligase IV Antibody (JM64-32) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human DNA Ligase IV aa 484-705 / 911. (SwissProt: P49917 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LIG4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 104 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for DNA Ligase IV Antibody (JM64-32)

• DNA joinase
• DNA ligase 4
• DNA Ligase IV
• DNA repair enzyme
• EC 6.5.1.1
• LIG4
• ligase IV, DNA, ATP-dependent
• Polydeoxyribonucleotide synthase [ATP] 4
• polynucleotide ligase
• sealase

Background

LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIG4