DKC1 Antibody

Western Blot: DKC1 Antibody [H00001736-B01P] - Analysis of DKC1 expression in A-431.

Immunocytochemistry/Immunofluorescence: DKC1 Antibody [H00001736-B01P] - Analysis of purified antibody to DKC1 on HeLa cell. (antibody concentration 10 ug/ml)

Immunohistochemistry-Paraffin: DKC1 Antibody [H00001736-B01P] - Analysis of purified antibody to DKC1 on formalin-fixed paraffin-embedded human salivary gland. (antibody concentration 3 ug/ml)

Western Blot: DKC1 Antibody [H00001736-B01P] - Analysis of DKC1 expression in transfected 293T cell line by DKC1 polyclonal antibody. Lane 1: DKC1 transfected lysate(56.54 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

DKC1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: DKC1 (NP_001354, 1 a.a. - 514 a.a.) full-length human protein. MADAEVIILPKKHKKKKERKSLPEEDVAEIQHAEEFLIKPESKVAKLDTSQWPLLLKNFDKLNVRTTHYTPLACGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVEKTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIEGGTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVMSEKDHMVTMHDVLDAQWLYDNHKDESYLRRVVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGVLRYEDGIEVNQEIVVITTKGEAICMAIALMTTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKKLMIKQGLLDKHGKPTDSTPATWKQEYVDYSESAKKEVVAEVVKAPQVVAEAAKTAKRKRESESESDETPPAAPQLIKKEKKKSKKDKKAKAGLESGAEPGDGDSDTTKKKKKKKKAKEVELVSE
• Specificity: DKC1 - dyskeratosis congenita 1, dyskerin,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: DKC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. May also be used for IF, IHC-P.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DKC1 Antibody

• CBF5 homolog
• CBF5
• cbf5p homolog
• DKC
• dyskeratosis congenita 1, dyskerin
• Dyskerin
• EC 5.4.99
• EC 5.4.99.-
• FLJ97620
• H/ACA ribonucleoprotein complex subunit 4
• NAP57
• NOLA4dyskerin
• Nopp140-associated protein of 57 kDa
• Nucleolar protein family A member 4
• Nucleolar protein NAP57
• snoRNP protein DKC1
• XAP101

Background

This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the NOLA1, 2 and 3 proteins. The protein encoded by this gene and the three NOLA proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The protein encoded by this gene is related to the Saccharomyces cerevisiae Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in a tail-to-tail orientation with the palmitoylated erythrocyte membrane protein gene and is transcribed in a telomere to centromere direction. Both nucleotide substitutions and single trinucleotide repeat polymorphisms have been found in this gene. Mutations in this gene cause X-linked dyskeratosis congenita, a disease resulting in reticulate skin pigmentation, mucosal leukoplakia, nail dystrophy, and progressive bone marrow failure in most cases. Mutations in this gene also cause Hoyeraal-Hreidarsson syndrome, which is a more severe form of dyskeratosis congenita. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DKC1
• Entrez:
  • 1736(Human)
• Uniprot:
  • NP_001354(Human)