Dishevelled-2 Overexpression Lysate (Native)


Western Blot: Dishevelled 2 Overexpression Lysate (Adult Normal) [NBL1-10063] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Dishevelled 2.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

Dishevelled-2 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC202233 and based on accession number NM_004422. The protein contains a C-terminal DDK tag.
Homo sapiens dishevelled, dsh homolog 2 (Drosophila) (DVL2), mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
78.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Dishevelled-2

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Dishevelled-2 Overexpression Lysate (Native)

  • dishevelled 2 (homologous to Drosophila dsh)
  • dishevelled, dsh homolog 2 (Drosophila)
  • Dishevelled2
  • Dishevelled-2
  • DSH homolog 2
  • DVL2
  • segment polarity protein dishevelled homolog DVL-2


This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Pm
Applications: WB
Species: Hu, Mu, Rt, Ca, Eq, Pm
Applications: WB, IHC-P
Species: Hu, Mu
Species: Hu, Mu, Rt
Applications: WB, IHC
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
Species: Hu
Applications: WB, IP, ICC
Species: Hu
Applications: WB
Species: Hu, Mu
Applications: WB
Species: Hu, Mu, Rt
Applications: WB
Species: Hu, Mu, Rt, Ch, Pm, Xp, Ze
Applications: WB, IHC-P
Species: Hu
Applications: WB, Flow, CyTOF-ready
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Bv, Ch, Eq, Op, Pm
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IP
Species: Hu
Species: Hu, Mu, Rt
Applications: WB, ICC
Species: Hu
Applications: WB, IHC

Publications for Dishevelled-2 Lysate (NBL1-10063) (0)

There are no publications for Dishevelled-2 Lysate (NBL1-10063).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Dishevelled-2 Lysate (NBL1-10063) (0)

There are no reviews for Dishevelled-2 Lysate (NBL1-10063). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Dishevelled-2 Lysate (NBL1-10063) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Dishevelled-2 Products

Bioinformatics Tool for Dishevelled-2 Lysate (NBL1-10063)

Discover related pathways, diseases and genes to Dishevelled-2 Lysate (NBL1-10063). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Dishevelled-2 Lysate (NBL1-10063)

Discover more about diseases related to Dishevelled-2 Lysate (NBL1-10063).

Pathways for Dishevelled-2 Lysate (NBL1-10063)

View related products by pathway.

PTMs for Dishevelled-2 Lysate (NBL1-10063)

Learn more about PTMs related to Dishevelled-2 Lysate (NBL1-10063).

Research Areas for Dishevelled-2 Lysate (NBL1-10063)

Find related products by research area.

Blogs on Dishevelled-2

There are no specific blogs for Dishevelled-2, but you can read our latest blog posts.

Customers Who Bought This Also Bought


Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Dishevelled-2 Overexpression Lysate (Native) and receive a gift card or discount.


Gene Symbol DVL2