DHPS Overexpression Lysate (Native)

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Western Blot: DHPS Overexpression Lysate (Adult Normal) [NBL1-09864] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DHPS.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DHPS Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204235 and based on accession number NM_001930. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens deoxyhypusine synthase (DHPS), transcript variant 1, mRNA.
Gene
DHPS

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
40.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for DHPS

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DHPS Overexpression Lysate (Native)

  • deoxyhypusine synthase
  • DHS
  • DS
  • EC 2.5.1
  • EC 2.5.1.46
  • MIG13
  • migration-inducing gene 13

Background

The unusual amino acid hypusine is formed posttranslationally and is only found in a single cellular protein, eukaryotic translation initiation factor 5A. In the first step of hypusine biosynthesis, deoxyhypusine synthase catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue. This gene consists of nine exons spanning 6.6 kb. Three transcript variants have been isolated. However, only transcript variant 1 encodes an active protein. The shorter variants may act as modulating factors of DHPS activity. [provided by RefSeq]. Transcript Variant: This splice variant (1) includes exon 7 and alternative exon 8a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for DHPS Lysate (NBL1-09864) (0)

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Bioinformatics

Gene Symbol DHPS