DGK-beta Antibody (OTI1F9)

Western Blot: DGKB Antibody (1F9) [NBP2-02733] Analysis of extracts (35ug) from 9 different cell lines by using anti-DGKB monoclonal antibody.

Western Blot: DGKB Antibody (1F9) [NBP2-02733] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKB.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB
• Clone: OTI1F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.54 mg/ml

DGK-beta Antibody (OTI1F9) Summary

• Description: Novus Biologicals Mouse DGK-beta Antibody (OTI1F9) (NBP2-02733) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human DGKB (NP_663733) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DGKB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-2000
• Theoretical MW: 86.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.54 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DGK-beta Antibody (OTI1F9)

• 90 kDa diacylglycerol kinase
• DAG kinase beta
• DAGK2
• DGKB
• DGKbeta
• DGK-beta
• diacylglycerol kinase beta
• diacylglycerol kinase, beta (90kD)
• diacylglycerol kinase, beta 90kDa
• Diglyceride kinase beta
• EC 2.7.1
• EC 2.7.1.107
• KIAA0718DGKDGK-BETA

Background

Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DGKB
• Entrez:
  • 1607(Human)