DGK-alpha Overexpression Lysate

Images

 
Western Blot: DGKA Overexpression Lysate (Adult Normal) [NBL1-09851] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DGKA.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DGK-alpha Overexpression Lysate Summary

Description
Transient overexpression lysate of diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 3 The lysate was created in HEK293T cells, using Plasmid ID RC222395 and based on accession number NM_001345. The protein contains a C-MYC/DDK Tag.
Gene
DGKA

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DGK-alpha Overexpression Lysate

  • DAG kinase alpha
  • DAGK
  • DAGK1
  • DAGK1diacylglycerol kinase, alpha (80kD)
  • DGKA
  • DGKalpha
  • DGK-alpha
  • DGK-alphaEC 2.7.1.107
  • diacylglycerol kinase alpha
  • diacylglycerol kinase, alpha 80kDa
  • Diglyceride kinase alpha
  • MGC12821,80 kDa diacylglycerol kinase
  • MGC42356

Background

The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq]. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for DGK-alpha Lysate (NBL1-09851) (0)

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Bioinformatics

Gene Symbol DGKA