DGCR8 Antibody

Product Discontinued

DGCR8 Antibody Summary

• Immunogen: Peptide with sequence C-KRFDFEQVTVKKFRT corresponding to internal region according to NP_073557.3.
• Epitope: C-KRFDFEQVTVKKFRT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DGCR8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 75 kDa band in human brain (amygdala) lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 86.0 kDa band according to NP_073557.3. The 75 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for DGCR8 Antibody

• C22orf12
• DGCRK6chromosome 22 open reading frame 12
• DiGeorge syndrome critical region 8
• DiGeorge syndrome critical region gene 8
• Gy1
• microprocessor complex subunit DGCR8
• pasha

Background

DiGeorge syndrome critical region 8 (DGCR8) is a component of the microprocessor complex that acts as a RNA- and heme-binding protein and is involved in the initial step of microRNA (miRNA) biogenesis. DGCR8 functions as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junctions. It directs RNASEN/DROSHA to cleave 11 bp away from the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DGCR8
• Entrez:
  • 54487(Human)
  • 94223(Mouse)
  • 287954(Rat)
• Uniprot:
  • NP_073557.3(Human)