Recombinant Human Destrin His Protein

SDS-Page: Recombinant Human Destrin Protein [NBP1-98933] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human Destrin His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-165 of Human Destrin
  
  

  Source: E.coli

  Amino Acid Sequence: MASGVQVADE VCRIFYDMKV RKCSTPEEIK KRKKAVIFCL SADKKCIIVE EGKEILVGDV GVTITDPFKH FVGMLPEKDC RYALYDASFE TKESRKEELM FFLWAPELAP LKSKMIYASS KDAIKKKFQG IKHECQANGP EDLNRACIAE KLGGSLIVAF EGCPVLEHHH HHH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DSTN
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 19.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Destrin His Protein

• ACTDPdestrin
• Actin-depolymerizing factor
• ADFbA462D18.2 (destrin (actin depolymerizing factor ADF) (ACTDP))
• bA462D18.2
• destrin (actin depolymerizing factor)
• DSN

Background

Destrin (actin depolymerizing factor), also known as DSTN, is a member of the ADF/Cofilin/destrin superfamily that has the ability to rapidly depolymerize F-Actin in a stoichiometric manner. DSTN is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It is found in various epithelial and endothelial cells but is practically absent from adult mouse heart and skeletal muscle cells. DSTN shares 71% sequence homology with Cofilin, however the two proteins differ in their interaction with Actin. Recombinant human DSTN protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DSTN