DDX6 Antibody (JB41-32)

Western Blot: DDX6 Antibody (JB41-32) [NBP2-75466] - Analysis of DDX6 on different lysates using anti-DDX6 antibody at 1/500 dilution.Positive control:Lane 1: Human colon Lane 2: SH-SY-5YLane 3: SiHa Lane 4: K562

Immunohistochemistry-Paraffin: DDX6 Antibody (JB41-32) [NBP2-75466] - Analysis of paraffin-embedded rat brain tissue using anti-DDX6 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: DDX6 Antibody (JB41-32) [NBP2-75466] - Analysis of paraffin-embedded human tonsil tissue using anti-DDX6 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: DDX6 Antibody (JB41-32) [NBP2-75466] - Analysis of paraffin-embedded human kidney tissue using anti-DDX6 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: DDX6 Antibody (JB41-32) [NBP2-75466] - Analysis of paraffin-embedded mouse small intestine tissue using anti-DDX6 antibody. Counter stained with hematoxylin.

Product Discontinued

DDX6 Antibody (JB41-32) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human DDX6 aa 81-220 / 483. (SwissProt: P26196 Human; SwissProt: P54823 Mouse)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DDX6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for DDX6 Antibody (JB41-32)

• ATP-dependent RNA helicase p54
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
• DEAD box protein 6
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
• EC 3.6.1
• EC 3.6.4.13
• FLJ36338
• HLR2DEAD box-6
• Oncogene RCK
• probable ATP-dependent RNA helicase DDX6
• RCKP54

Background

DDX6 encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX6