DARS Antibody

Images

 
Western Blot: DARS Antibody [NBP1-33689] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 C: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: DARS Antibody [NBP1-33689] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: DARS Antibody [NBP1-33689] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.35 mg/ml

Order Details

DARS Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DARS. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DARS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (90%) and Xenopus tropicalis (90%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.35 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DARS Antibody

  • aspartate tRNA ligase 1, cytoplasmic
  • Aspartate--tRNA ligase
  • aspartyl-tRNA synthetase
  • aspartyl-tRNA synthetase, cytoplasmic
  • aspRS
  • Cell proliferation-inducing gene 40 protein
  • cell proliferation-inducing protein 40
  • DKFZp781B11202
  • EC 6.1.1.12
  • MGC111579

Background

Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for DARS Antibody (NBP1-33689)

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Calculators

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Bioinformatics

Gene Symbol DARS