DARPP-32 Antibody (EP720Y)

Product Discontinued

DARPP-32 Antibody (EP720Y) Summary

• Immunogen: A synthetic peptide corresponding to residues near the N-terminal of human DARPP-32 was used as immunogen.
• Marker: Neuronal Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PPP1R1B
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:7500
  • Immunohistochemistry-Paraffin 1:250-500
  • Immunoprecipitation 1:50
  • Western Blot 1: 5000-50000
• Application Notes: In Western blot this antibody detects a band at approximately 32kDa.
  Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:50-1:16000; matrix was 12-230 kDa.
• Control:

  DARPP-32 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for DARPP-32 Antibody (EP720Y)

• DARPP32
• DARPP-32
• PPP1R1B
• protein phosphatase 1, regulatory (inhibitor) subunit 1B
• regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Background

The 32kDa dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated (1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1 (2-3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5(Cdk5), DARPP-32 is converted into an inhibitor of PKA (1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DARPP-32 Antibody (NB100-79931)(2)

Publications using NB100-79931

• Reinius B, Blunder M, Brett FM et al. Conditional targeting of medium spiny neurons in the striatal matrix. Front Behav Neurosci 2015-01-01 [PMID: 25870547] (IF/IHC)
• Bode FJ, Stephan M, Suhling H et al. Sex differences in a transgenic rat model of Huntington's disease: decreased 17beta-estradiol levels correlate with reduced numbers of DARPP32+ neurons in males. Hum Mol Genet 2008-09-01 [PMID: 18502785]

Bioinformatics

• Gene Symbol: PPP1R1B
• Entrez:
  • 84152(Human)
  • 19049(Mouse)
  • 360616(Rat)
• Uniprot:
  • Q9UD71(Human)