Cytosolic Sulfotransferase 2B1/SULT2B1 Overexpression Lysate

Images

 
Western Blot: SULT2B1 Overexpression Lysate (Adult Normal) [NBL1-16608] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SULT2B1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Cytosolic Sulfotransferase 2B1/SULT2B1 Overexpression Lysate Summary

Description

Cytosolic Sulfotransferase 2B1/SULT2B1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC204478

Accession#: NM_177973

Protein Tag: C-MYC/DDK
Gene
SULT2B1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
41.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Cytosolic Sulfotransferase 2B1/SULT2B1 Overexpression Lysate

  • Alcohol sulfotransferase
  • Cytosolic Sulfotransferase 2B1
  • EC 2.8.2
  • EC 2.8.2.2
  • HSST2
  • HSST2sulfotransferase family cytosolic 2B member 1
  • Hydroxysteroid sulfotransferase 2
  • ST2B1
  • Sulfotransferase 2B1
  • sulfotransferase family, cytosolic, 2B, member 1
  • SULT2B1

Background

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene sulfates dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode different isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (2) is also known as SULT2B1b. It is the predominant transcript and it encodes the longer protein (isoform b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Cytosolic Sulfotransferase 2B1/SULT2B1 Lysate (NBL1-16608) (0)

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Bioinformatics

Gene Symbol SULT2B1