Cytokeratin, LMW Antibody (KRTL/4440R) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: IHC
• Clone: KRTL/4440R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

Cytokeratin, LMW Antibody (KRTL/4440R) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide corresponding to Cytokeratin, LMW residues within aa100-200 of Cytokeratin, LMW (Uniprot: Q7Z794)
• Localization: Cytoplasmic
• Marker: Epithelial Marker
• Specificity: The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. It usually used in a cytokeratin cocktail with type II cytokeratin antibody.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT77
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Cytokeratin, LMW Antibody (KRTL/4440R) [CoraFluor™ 1]

• Cytokeratin LMW
• cytokeratin-1B
• keratin 77
• keratin, type II cytoskeletal 1b
• KRT77
• type-II keratin Kb39

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT77