Human epidermal keratin was used as the immunogen for the Cytokeratin HMW AE3 antibody.
This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Protein G purified
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Protein G purified
Western Blot 0.5-1ug/ml
ELISA 1-5ug/ml for coating
Flow Cytometry 0.5-1ug/million cells
Human Chromosome Location: 12q13.13 Mol. Weight of Antigen: 52-67kDa Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Clone AE3 has been extensively characterized and cited in the scientific literature since the 1980's. Immunocytochemistry/Immunofluorescence, Western Blot and Immunohistochemistry-Frozen applications reported in literature.
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Alternate Names for Cytokeratin, HMW Antibody (AE3)
keratin, type II cytoskeletal 2 oral
Type-II keratin Kb9
Among the cytoplasmic intermediate filaments (IF) proteins, keratins make up the largest family and are expressed specifically in epithelial cells in a cell-specific manner. Keratins include more than 20 unique gene products (termed K1-K20) that are divided into type I (K9-K20) and type II (K1-K8). Most epithelial cells express at least one type I and one type II keratin as their predominant IF protein complement in an epithelial cell-specific manner. Most of the type I cytokeratin consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. and the type II cytokeratin are clustered in a region of chromosome 12q12-q13.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.