Cytokeratin 6 Antibody (LHK6 (same as LHK6B)) [DyLight 405]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-Fr, IHC-P
Clone
LHK6 (same as LHK6B)
Clonality
Monoclonal
Host
Mouse

Cytokeratin 6 Antibody (LHK6 (same as LHK6B)) [DyLight 405] Summary

Immunogen
A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from C-terminus of human keratin 6
Localization
Cytoplasmic
Specificity
This MAb recognizes a protein of 56kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT6A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 405
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Immunohistochemistry (Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 10-15 min at RT)
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Alternate Names for Cytokeratin 6 Antibody (LHK6 (same as LHK6B)) [DyLight 405]

  • Allergen Hom s 5
  • CK6A
  • CK-6A
  • CK6C
  • CK6D
  • CK-6D
  • cytokeratin 6A
  • cytokeratin 6C
  • cytokeratin 6D
  • Cytokeratin-6A
  • Cytokeratin-6D
  • K6A
  • K6C
  • K6D
  • keratin 6A
  • keratin 6C
  • keratin 6D
  • keratin, epidermal type II, K6A
  • keratin, type II cytoskeletal 6A
  • Keratin-6A
  • KRT6C
  • KRT6D
  • Type-II keratin Kb6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRT6A

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