Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free

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Product Details

Summary
Reactivity Hu, Rt, Po, Bv, GtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
SPM560
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free Summary

Immunogen
The cytoskeletal fraction of rat colon epithelium
Localization
Cytoplasmic
Marker
Basal Epithelial Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT17
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug
  • CyTOF-ready
Application Notes
Immunohistochemistry (Staining of paraffin tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS - BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Cytokeratin 17 Antibody (SPM560) - Azide and BSA Free

  • 39.1
  • CK-17
  • cytokeratin-17
  • K17
  • keratin 17
  • keratin, type I cytoskeletal 17
  • keratin-17
  • PC
  • PC2
  • PCHC1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRT17