Cytochrome P450 26B1 Overexpression Lysate (Native)

Images

 
Western Blot: Cytochrome P450 26B1 Overexpression Lysate (Adult Normal) [NBL1-09680] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cytochrome P450 26B1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Cytochrome P450 26B1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC221075 and based on accession number NM_019885. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cytochrome P450, family 26, subfamily B, polypeptide 1 (CYP26B1), mRNA.
Gene
CYP26B1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBL1-09680.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Cytochrome P450 26B1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Cytochrome P450 26B1 Overexpression Lysate (Native)

  • CYP26A2
  • Cytochrome P450 26A2
  • cytochrome P450 26B1
  • cytochrome P450 CYP26B1 variant 2
  • Cytochrome P450 retinoic acid-inactivating 2
  • cytochrome P450 retinoid metabolizing protein
  • cytochrome P450, family 26, subfamily B, polypeptide 1
  • Cytochrome P450RAI-2
  • DKFZp686G0638
  • EC 1.14
  • MGC129613
  • P450RAI2
  • P450RAI-2
  • Retinoic acid-metabolizing cytochrome

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and the synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene is involved in the specific inactivation of all-trans-retinoic acid to hydroxylated forms, such as 4-oxo-, 4-OH-, and 18-OH-all-trans-retinoic acid. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Cytochrome P450 26B1 Lysate (NBL1-09680)(1)

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol CYP26B1