Cystatin SN Antibody Pair [HRP]

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Sandwich ELISA: Cystatin SN Antibody Pair [HRP] [NBP2-79654] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Clonality
Monoclonal
Host
Rabbit
Conjugate
HRP
Standard Curve Range
23.44-1500 pg/ml (example only; lot dependent)
Sensitivity
23.44 pg/ml (example only; lot dependent)

Order Details

Cystatin SN Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human Cystatin SN.
Additional Information
Recombinant Monoclonal Antibody
Specificity
This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Cystatin SN.
Standard Curve Range
23.44-1500 pg/ml (example only; lot dependent)
Sensitivity
23.44 pg/ml (example only; lot dependent)
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Clonality
Monoclonal
Host
Rabbit
Gene
CST1

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
Application Notes
1. Capture Antibody (NBP2-89918): Dilute to a working concentration of 2 ug/ml in PBS before coating.
2. Capture Antibody (NBP2-89919): Dilute to working concentration of 0.5 ug/ml in detection antibody dilution buffer before use.
3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1500 pg/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Capture Antibody: 1 mg/ml of rabbit anti-Human Cystatin SN monoclonal antibody (in PBS, pH 7.4).
  2. Detection Antibody: 0.2 mg/ml of rabbit anti-Human Cystatin SN monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  3. Standard: Each vial contains 62 ng of recombinant Human Cystatin SN.

Alternate Names for Cystatin SN Antibody Pair [HRP]

  • CST1
  • cystain-SA-I
  • cystatin 1
  • cystatin SA-I
  • Cystatin SN
  • cystatin-1
  • cystatin-SN
  • Salivary cystatin-SA-1
  • type 2 family

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cystatin SN Antibody Pair (NBP2-79654). (Showing 1 - 1 of 1 FAQ).

  1. I notice you sell both antibodies and recombinant protein for human cystatin SN. Could you perhaps develop an ELISA for this protein?
    • NBP1-55995 and H00001469-B01P are both suitable for use as antibodies in ELISA. While a kit is not in development, you could use these to make your own ELISA kit, as many of our users do.

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Bioinformatics

Gene Symbol CST1