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Cyr61/CCN1 Antibody

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Western Blot: Cyr61/CCN1 Antibody [NB100-358] - Detection of cleaved CYR61 in MDA-MB-231 cell lysate using NB 100-358. Photo courtesy of Dr. Lupu's Lab, Northwestern University. Image by Dr. Ingrid Espinoza.
Western Blot: Cyr61/CCN1 Antibody [NB100-358] - Detection of cleaved CYR61 in MDA-MB-231 cell lysate using NB 100-358. Photo courtesy of Dr. Lupu's Lab, Northwestern University. Image by Dr. Ingrid Espinoza.
Immunocytochemistry/ Immunofluorescence: Cyr61/CCN1 Antibody [NB100-358] - CYR61 antibody was tested in MCF-7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, Ch, XpSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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Cyr61/CCN1 Antibody Summary

Immunogen
A synthetic peptide made to the C-terminal region of human CYR61. [UniProt# O00622]
Predicted Species
Xenopus (99%), Chicken (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCN1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:200
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
Application Notes
This CYR61 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at both ~37 kDa (processed form) and ~45 kDa (full-length form) of CYR61 protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Cyr61/CCN1 Overexpression Lysate
Cyr61/CCN1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-358 in the following applications:

Reactivity Notes

Immunogen sequence has 99% homology to Xenopus, 93% homology to chicken and 80% homology to rat and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2), 1.0% BSA, and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cyr61/CCN1 Antibody

  • CCN family member 1
  • CCN1
  • CCN1IGF-binding protein 10
  • Cyr61
  • Cysteine-rich angiogenic inducer 61
  • cysteine-rich, angiogenic inducer, 61
  • GIG1
  • GIG1cysteine-rich heparin-binding protein 61
  • IBP-10
  • IGFBP-10
  • IGFBP10cysteine-rich, anigogenic inducer, 61
  • Insulin-like growth factor-binding protein 10
  • protein CYR61
  • Protein GIG1

Background

CYR61 (cysteine-rich angiogenic inducer 61), a heparin-binding growth factor, belongs to CCN family whose members are: cysteine-rich 61 (CYR61/CCN1), connective tIssue growth factor (CTGF/CCN2), nephroblastoma overexpressed (NOV/CCN3), and Wnt-induced secreted proteins-1 (WISP-1/CCN4), -2 (WISP-2/CCN5) and -3 (WISP-3/CCN6). CYR61 localizes intracellularly or associates with extracellular matrix at cell surfaces and its reported nuclear localization is surprising, since the primary structure of CYR61 does not include a classic nuclear localization sequence, however, like NOV, EGF, FGF, PDGF, angiogenin, PTHrP etc which lack afore-mentioned sequence, CYR61's nuclear presence may be correlated with transcriptional regulation, and/or mRNA transport. CYR61 plays a key role in wound healing by up-regulating the expression of several genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1, integrins alpha-3 and alpha-5. CYR61 down-regulates the expression of alpha-1/alpha-2 subunits of collagen type-1 and promotes cell adhesion/adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. CYR61 has been reported to exert its diverse functions via cross-talk with/activating of Wnt, NFkB, AKT and tyrosine kinase signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Cyr61/CCN1 Antibody
NB100-358
Cyr61/CCN1 Antibody
Species: Hu, Ch, Xp
Applications: WB, ICC/IF

Publications for Cyr61/CCN1 Antibody (NB100-358) (0)

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Review for Cyr61/CCN1 Antibody (NB100-358) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-358:
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reviewed by:
chandra kurapaty
 WB Human 04/29/2014
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Summary

ApplicationWestern Blot
Sample TestedWHOLE CELL LYSATE, MEDIUM CONDITIONED
SpeciesHuman

Blocking

Blocking Details5% milk in TBST

Primary Anitbody

Dilution Ratio1:1000, o/n, 4c, 4% BSA in TBST

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP-linked Antibody
Secondary Manufacturer Cat#7074
Secondary Concentration1:1000

Details

Detection NotesECL

Product General Protocols

View specific protocols for Cyr61/CCN1 Antibody (NB100-358): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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FAQs for Cyr61/CCN1 Antibody (NB100-358) (0)

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Control Lysate(s)

Cyr61/CCN1 Overexpression Lysate
Cyr61/CCN1 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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 chandra kurapaty
04/29/2014
Application: WB
Species: Human
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Bioinformatics

Gene Symbol CCN1
Entrez
Uniprot
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