Western Blot: Cyr61/CCN1 Antibody [NB100-358] - Detection of cleaved CYR61 in MDA-MB-231 cell lysate using NB 100-358. Photo courtesy of Dr. Lupu's Lab, Northwestern University. Image by Dr. Ingrid Espinoza.
Immunocytochemistry/ Immunofluorescence: Cyr61/CCN1 Antibody [NB100-358] - CYR61 antibody was tested in MCF-7 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
This CYR61 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at both ~37 kDa (processed form) and ~45 kDa (full-length form) of CYR61 protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Immunogen sequence has 99% homology to Xenopus, 93% homology to chicken and 80% homology to rat and mouse.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS (pH 7.2), 1.0% BSA, and 50% Glycerol
0.09% Sodium Azide
Immunogen affinity purified
Alternate Names for Cyr61/CCN1 Antibody
CCN family member 1
CCN1IGF-binding protein 10
Cysteine-rich angiogenic inducer 61
cysteine-rich, angiogenic inducer, 61
GIG1cysteine-rich heparin-binding protein 61
IGFBP10cysteine-rich, anigogenic inducer, 61
Insulin-like growth factor-binding protein 10
CYR61 (cysteine-rich angiogenic inducer 61), a heparin-binding growth factor, belongs to CCN family whose members are: cysteine-rich 61 (CYR61/CCN1), connective tIssue growth factor (CTGF/CCN2), nephroblastoma overexpressed (NOV/CCN3), and Wnt-induced secreted proteins-1 (WISP-1/CCN4), -2 (WISP-2/CCN5) and -3 (WISP-3/CCN6). CYR61 localizes intracellularly or associates with extracellular matrix at cell surfaces and its reported nuclear localization is surprising, since the primary structure of CYR61 does not include a classic nuclear localization sequence, however, like NOV, EGF, FGF, PDGF, angiogenin, PTHrP etc which lack afore-mentioned sequence, CYR61's nuclear presence may be correlated with transcriptional regulation, and/or mRNA transport. CYR61 plays a key role in wound healing by up-regulating the expression of several genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1, integrins alpha-3 and alpha-5. CYR61 down-regulates the expression of alpha-1/alpha-2 subunits of collagen type-1 and promotes cell adhesion/adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. CYR61 has been reported to exert its diverse functions via cross-talk with/activating of Wnt, NFkB, AKT and tyrosine kinase signaling pathways.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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