CYLD Antibody

Images

 
Western Blot: CYLD Antibody [NB110-95574] - Detection of CYLD in Jurkat whole cell extract using NB110-95574.
Immunocytochemistry/ Immunofluorescence: CYLD Antibody [NB110-95574] - CYLD antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Summary
Reactivity Hu, Mu, Bv, Pm, Rt(-)Species Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CYLD Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human CYLD (within residues 500-600). [Swiss-Prot# Q9NQC7]
Localization
Cytoplasm, perinuclear region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYLD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:500 - 1:2000
  • Western Blot 2 ug/ml
Application Notes
This CYLD antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a doublet is seen ~120 kDa (Hellerbrand, et al.) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Jurkat Whole Cell Lysate
Publications
Read Publication using NB110-95574.

Reactivity Notes

Orangutan. Does not react with rat.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CYLD Antibody

  • BRSS
  • CDMT
  • CYLD
  • CYLD1
  • CYLD1MFT
  • CYLDI
  • cylindromatosis (turban tumor syndrome)
  • Deubiquitinating enzyme CYLD
  • EAC
  • EC 3.1.2.15
  • EC 3.4.19.12
  • FLJ20180
  • FLJ31664
  • KIAA0849FLJ78684
  • MFT1
  • probable ubiquitin carboxyl-terminal hydrolase CYLD
  • SBS
  • TEM
  • ubiquitin carboxyl-terminal hydrolase CYLD
  • ubiquitin specific peptidase like 2
  • ubiquitin thioesterase CYLD
  • Ubiquitin thiolesterase CYLD
  • Ubiquitin-specific-processing protease CYLD
  • USPL2

Background

CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-540
Species: Ec, Mu
Applications: CyTOF-ready, Flow-IC, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
H00003930-B01P
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
NBP1-97768
Species: Hu, Mu, Rt
Applications: PEP-ELISA, WB
NB100-56173
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
H00004627-M03
Species: Bv, Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP2-26506
Species: Hu, Mu, Rt
Applications: B/N, In vitro
NBP2-25200
Species: Hu
Applications: B/N, Flow, IHC, IHC-Fr, WB
NBP2-26504
Species: Hu, Mu, Rt
Applications: B/N, Func-Inh, In vitro, In vivo
NBP1-45210
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NB100-56144
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
AF1067
Species: Hu, Mu, Rt
Applications: ELISA, IHC, Neut, WB
MAB17761
Species: Hu, Mu, Rt
Applications: ICC, WB
7770-GT
Species: Hu
Applications: EnzAct
NBP2-93808
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MAB41681
Species: Hu
Applications: WB
NB110-95574
Species: Hu, Mu, Bv, Pm, Rt(-)
Applications: WB, ICC/IF

Publications for CYLD Antibody (NB110-95574)(1)

We have publications tested in 1 confirmed species: Human.


Filter By Application
All Applications
Filter By Species
Human
(1)
All Species

Reviews for CYLD Antibody (NB110-95574) (0)

There are no reviews for CYLD Antibody (NB110-95574). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for CYLD Antibody (NB110-95574): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CYLD Antibody (NB110-95574) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional CYLD Products

Diseases for CYLD Antibody (NB110-95574)

Discover more about diseases related to CYLD Antibody (NB110-95574).
 

Pathways for CYLD Antibody (NB110-95574)

View related products by pathway.

PTMs for CYLD Antibody (NB110-95574)

Learn more about PTMs related to CYLD Antibody (NB110-95574).
 

Research Areas for CYLD Antibody (NB110-95574)

Find related products by research area.

Blogs on CYLD

There are no specific blogs for CYLD, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CYLD Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CYLD
Entrez
Uniprot