Cyclophilin B Antibody (CL3901) - BSA Free

Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis in human placenta and skeletal muscle tissues using NBP2-59781 antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.

Western Blot: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-PPIB monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Western Blot: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human skeletal muscle shows negative to very weak cytoplasmic positivity in myocytes.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, Mycoplasma
• Clone: CL3901
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Cyclophilin B Antibody (CL3901) - BSA Free Summary

• Description: Novus Biologicals Mouse Cyclophilin B Antibody (CL3901) - BSA Free (NBP2-59781) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from P23284, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPIB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Cyclophilin B Antibody (CL3901) - BSA Free

• Cyclophilin B
• cyclophilin-like protein
• CYPB
• CYPBEC 5.2.1.8
• CYP-S1
• MGC2224
• OI9MGC14109
• peptidyl-prolyl cis-trans isomerase B
• peptidylprolyl isomerase B (cyclophilin B)
• PPIase B
• PPIB
• Rotamase B
• S-cyclophilin
• SCYLP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPIB