Cyclophilin B Antibody (CL3901) - Azide and BSA Free

Analysis in human placenta and skeletal muscle tissues using NBP3-44066 antibody. Corresponding Cyclophilin B RNA-seq data are presented for the same tissues.

Staining of human skeletal muscle shows negative to very weak cytoplasmic positivity in myocytes.

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Cyclophilin B antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL3901
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Cyclophilin B Antibody (CL3901) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a synthetic peptide of P23284, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPIB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Cyclophilin B Antibody (CL3901) - Azide and BSA Free

• Cyclophilin B
• cyclophilin-like protein
• CYPB
• CYPBEC 5.2.1.8
• CYP-S1
• MGC2224
• OI9MGC14109
• peptidyl-prolyl cis-trans isomerase B
• peptidylprolyl isomerase B (cyclophilin B)
• PPIase B
• PPIB
• Rotamase B
• S-cyclophilin
• SCYLP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPIB