Cyclophilin A Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyclophilin A at approximately 25 ...read more
Cyclophilin A was detected in immersion fixed PANC-1 human pancreatic carcinoma cell line using Goat Anti-Human/Mouse/Rat Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589) at 10 µg/mL for 3 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Cyclophilin A Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Cyclophilin A
Met1-Glu165
Accession # P62937
Specificity
Detects human, mouse, and rat Cyclophilin A in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PPIA
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Simple Western 5 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publication using
AF3589 in the following applications:

  • IF
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyclophilin A Antibody [Unconjugated]

  • Cyclophilin A
  • Cyclosporin A-binding protein
  • CYPA
  • CYPAMGC12404
  • CYPH
  • EC 5.2.1.8
  • MGC117158
  • MGC23397
  • peptidyl-prolyl cis-trans isomerase A
  • peptidylprolyl isomerase A (cyclophilin A)
  • PPIA
  • PPIase A
  • Rotamase A
  • Rotamase
  • T cell cyclophilin

Background

Cyclophilin A, also called Peptidyl-prolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis- and trans- proline residues critical for proper folding of proteins (1). Cyclophilin is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity (2). The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity (3) and prevent incorporation of cyclophilin into viral particles (4). The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin (3, 5), making it a useful inhibitor for studying calcineurin activity.

  1. Hamilton, G.S. and J.P. Steiner  (1998) J. Med. Chem. 41:5119.
  2. Cantin, R. et al. (2005) J. Virology 79:6577.
  3. Liu, J. et al. (1992) Biochemistry 31:3896.
  4. Wiegers K. and H.G. Krausslich (2002) Virology 294:289.
  5. Liu, J. et al. (1991) Cell 66:807.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cyclophilin A Antibody (AF3589)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IF.


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Bioinformatics

Gene Symbol PPIA
Entrez
Uniprot