Cyclin E2 Antibody (SD2035)

Immunocytochemistry/Immunofluorescence: Cyclin E2 Antibody (SD2035) [NBP2-67744] - Staining Cyclin E2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Cyclin E2 Antibody (SD2035) [NBP2-67744] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cyclin E2 antibody. Counter stained with hematoxylin.

Flow Cytometry: Cyclin E2 Antibody (SD2035) [NBP2-67744] - Analysis of Hela cells with Cyclin E2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Cyclin E2 Antibody (SD2035) [NBP2-67744] - Staining Cyclin E2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Cyclin E2 Antibody (SD2035) [NBP2-67744] - Staining Cyclin E2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Cyclin E2 Antibody (SD2035) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within C-terminal human Cyclin E2. (SwissProt: O96020 Human; SwissProt: Q7TMS8 Mouse; SwissProt: Q9Z238 Mouse)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CCNE2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cyclin E2 Antibody (SD2035)

• CCNE2
• CYCE2
• Cyclin E2
• G1/S-specific cyclin E2
• G1/S-specific cyclin-E2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCNE2