| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7444-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-130SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99464-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99464-100ul | |||
| Azide and BSA Free | NBP2-99464-50ul | |||
![Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-27820241631678.jpg "Western Blot: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: HEK-293 cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 51/47 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32237) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32237) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-29820241435260.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclin E2 Antibody (40-89-R) [NBP3-32237] - Immunocytochemistry analysis of HeLa cells labeling Cyclin E2 with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Cyclin E2 antibody (NBP3-32237) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32237_mouse-cyclin-e2-mab-40-89-r-292024119231.jpg)
![Immunoprecipitation:Cyclin E2 AntibodyNBP3-29686] -Detection of human CCNE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCNE2 antibody NBP3-29686 (lot NBP3-29686-1) used for IP at 6 µg per reaction. CCNE2 was also immunoprecipitated by rabbit anti-CCNE2 antibody. For blotting immunoprecipitated CCNE2, NBP3-29686 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29686_rabbit-cyclin-e2-pab-301220241952587.jpg "Immunoprecipitation:Cyclin E2 AntibodyNBP3-29686] -Detection of human CCNE2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCNE2 antibody NBP3-29686 (lot NBP3-29686-1) used for IP at 6 µg per reaction. CCNE2 was also immunoprecipitated by rabbit anti-CCNE2 antibody. For blotting immunoprecipitated CCNE2, NBP3-29686 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Cyclin E2 Antibody [NBP1-77755] - shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown).](http://images.novusbio.com/fullsize/Cyclin-E2-Antibody-Western-Blot-NBP1-77755-img0001.jpg "Western Blot: Cyclin E2 Antibody [NBP1-77755] - shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown).")
![Western Blot: Cyclin E2 Antibody [NB500-130] - Analysis of Cyclin E2 in CT10 cell line using this antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Cyclin-E2-Antibody-Western-Blot-NB500-130-img0002.jpg "Western Blot: Cyclin E2 Antibody [NB500-130] - Analysis of Cyclin E2 in CT10 cell line using this antibody at 1:500 dilution.")
![ELISA: Rat Cyclin E2 ELISA Kit (Colorimetric) [NBP2-75111] - Samples were spiked with high concentrations of Rat Cyclin E2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75111_rat-cyclin-e2-elisa-kit-colorimetric-13220241681434.jpg "ELISA: Rat Cyclin E2 ELISA Kit (Colorimetric) [NBP2-75111] - Samples were spiked with high concentrations of Rat Cyclin E2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Cyclin E2 ELISA Kit (Colorimetric) [NBP2-75111] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75111_rat-cyclin-e2-elisa-kit-colorimetric-432024857436.jpg)
![ELISA: Mouse Cyclin E2 ELISA Kit (Colorimetric) [NBP2-75110] - Samples were spiked with high concentrations of Mouse Cyclin E2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75110_mouse-cyclin-e2-elisa-kit-colorimetric-132202416225271.jpg "ELISA: Mouse Cyclin E2 ELISA Kit (Colorimetric) [NBP2-75110] - Samples were spiked with high concentrations of Mouse Cyclin E2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Cyclin E2 ELISA Kit (Colorimetric) [NBP2-75110] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75110_mouse-cyclin-e2-elisa-kit-colorimetric-432024856799.jpg)
![Western Blot: Cyclin E2 Overexpression Lysate (Adult Normal) [NBL1-08878] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cyclin E2.](http://images.novusbio.com/fullsize/Cyclin-E2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08878-img0002.jpg "Western Blot: Cyclin E2 Overexpression Lysate (Adult Normal) [NBL1-08878] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cyclin E2.")
![Western Blot: Cyclin E2 Antibody (0Y7G5) [NBP3-16806] - Western blot analysis of extracts of various cell lines, using Cyclin E2 Rabbit mAb (NBP3-16806) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Cyclin-E2-Antibody-0Y7G5-Western-Blot-NBP3-16806-img0001.jpg "Western Blot: Cyclin E2 Antibody (0Y7G5) [NBP3-16806] - Western blot analysis of extracts of various cell lines, using Cyclin E2 Rabbit mAb (NBP3-16806) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Cyclin E2 Antibody [NBP2-99464] - Immunofluorescence staining of Cyclin E2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human Cyclin E2 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.](http://images.novusbio.com/fullsize/Cyclin-E2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99464-img0001.jpg "Immunocytochemistry/Immunofluorescence: Cyclin E2 Antibody [NBP2-99464] - Immunofluorescence staining of Cyclin E2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human Cyclin E2 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.")