Cyclin A2 Antibody

Images

 
Western Blot: Cyclin A2 Antibody [NB600-495] - Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 ug per lane. ...read more
Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NB600-495] - Anti-Cyclin A antibody was diluted 1:500 to detect Cyclin A in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Cyclin A2 Antibody Summary

Description
This antibody was prepared from monospecific antiserum by delipidation and defibrination.
Immunogen
Fusion protein (Human).
Specificity
Antiserum will specifically react with a 60 kDa Cyclin A protein. No reaction was observed against other related cyclins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCNA2
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:5000-1:20000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
Application Notes
This product was assayed by immunoblot and found to be reactive against Cyclin A at a dilution of 1:250 - 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Anti-Cyclin A is suitable for the detection by immunoblot of human, rat and mouse Cyclin A. Suggested dilutions for ELISA are 1:500 - 1:2,000.
Positive Control
Cyclin A2 Lysate (NBL1-08864)

Reactivity Notes

Cross-reacts with Human, Rat and Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Antisera (Concentration 80 mg/ml by Refractometry)
Preservative
0.01% Sodium Azide
Purity
Delipidation and Defibrination

Alternate Names for Cyclin A2 Antibody

  • CCN1CCNAcyclin-A
  • CCNA
  • CCNA2
  • Cyclin A2
  • Cyclin-A
  • cyclin-A2

Background

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2 phase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CCNA2
Entrez