CUGBP1/CELF1 Antibody (7A1-R)

Western Blot: CUGBP1/CELF1 Antibody (7A1-R) [NBP3-32179] - Western blot analysis of CUGBP1/CELF1 on different lysates with Mouse anti-CUGBP1/CELF1 antibody (NBP3-32179) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: NCI-H226 cell lysate Lane 4: COS-1 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32179) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CUGBP1/CELF1 Antibody (7A1-R) [NBP3-32179] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-CUGBP1/CELF1 antibody (NBP3-32179) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32179) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: CUGBP1/CELF1 Antibody (7A1-R) [NBP3-32179] - Immunocytochemistry analysis of HeLa cells labeling CUGBP1/CELF1 with Mouse anti-CUGBP1/CELF1 antibody (NBP3-32179) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CUGBP1/CELF1 antibody (NBP3-32179) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clone: 7A1-R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CUGBP1/CELF1 Antibody (7A1-R) Summary

• Description: Novus Biologicals Mouse CUGBP1/CELF1 Antibody (7A1-R) (NBP3-32179) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human CUGBP1/CELF1 aa 1-200 / 486. (Uniprot: Q92879)
• Localization: Cytoplasm. Nucleus.
• Source: HEK293
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CELF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CUGBP1/CELF1 Antibody (7A1-R)

• BRUNOL2
• BRUNOL250 kDa nuclear polyadenylated RNA-binding protein
• bruno-like 2
• Bruno-like protein 2
• CELF1
• CUG RNA-binding protein
• CUG triplet repeat, RNA binding protein 1
• CUG triplet repeat, RNA-binding protein 1
• CUG-BP- and ETR-3-like factor 1
• CUGBP Elav-like family member 1
• CUG-BP
• CUGBP, Elav-like family member 1
• CUGBP1
• CUG-BP1
• CUGBP1CUG triplet repeat RNA-binding protein 1
• CUGBPCELF-1
• D2Wsu101e
• Deadenylation factor CUG-BP
• EDEN-BP homolog
• EDEN-BP
• embryo deadenylation element binding protein
• Embryo deadenylation element-binding protein homolog
• HNAB50
• NAB50
• NAB50CUG-BP1
• NAPOR
• nuclear polyadenylated RNA-binding protein, 50-kD
• RNA-binding protein BRUNOL-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CELF1