CTHRC1 Antibody

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse CTHRC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5960) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CTHRC1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

CTHRC1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Mouse CTHRC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5960) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CTHRC1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CTHRC1
  Ser33-Lys245
  Accession # Q3UAP6
• Specificity: Detects mouse CTHRC1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CTHRC1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CTHRC1 Antibody

• collagen triple helix repeat containing 1
• collagen triple helix repeat-containing protein 1
• CTHRC1
• NMTC1
• Protein NMTC1

Background

CTHRC1 (Collagen triple helix repeat-containing protein 1) is a 28-30 kDa, secreted glycoprotein that bears similarity to the C1q/TNF alpha -related family of proteins. It is expressed by disparate cell types, including renal epithelium, neurons, osteoblasts, and smooth muscle cells. Functionally, it is recognized to be induced by BMP-2 and to block TGF beta -induced collagen type I and III synthesis. Mouse CTHRC1 is 245 amino acids in length. It contains a 32 amino acid (aa) signal sequence, a 16 aa prosegment (aa 33-48), and a 197 aa mature region that shows one collagen-like domain (aa 59-92). Proteolytic processing may generate multiple CTHRC1 isoforms. There is the potential for an intracellular full-length 33 kDa, 245 aa form, plus extracellular isoforms that are 28 kDa (aa 33-245), 26 kDa (aa 49-245), 20 kDa (aa 98‑245) and 18 kDa and 16 kDa in size, the last two representing variants of the 20 kDa form with C-terminal processing. CTHRC1 may undergo dimerization, trimerization and oligomerization. One mouse splice variant shows a deletion of aa 53-126. Full-length mouse CTHRC1 shares 99% and 93% aa sequence identity with rat and human CTHRC1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CTHRC1 Antibody (AF5960)(2)

Publications using AF5960

• Qing Zhou, Wei Xiong, Xing Zhou, Rui‑Song Gao, Qun‑Fang Lin, Hui‑Ying Liu et al. CTHRC1 and PD‑1/PD‑L1 expression predicts tumor recurrence in prostate cancer Molecular Medicine Reports 2019-09-19 [PMID: 31545446]
• Qing Zhou, Wei Xiong, Xing Zhou, Rui‑Song Gao, Qun‑Fang Lin, Hui‑Ying Liu et al. CTHRC1 and PD‑1/PD‑L1 expression predicts tumor recurrence in prostate cancer Molecular Medicine Reports 2019-09-19 [PMID: 31545446] (Immunohistochemistry, Western Blot, Human)

Bioinformatics

• Gene Symbol: CTHRC1
• Uniprot:
  • Q3UAP6()