CSTF3 Antibody (1D4) - Azide and BSA Free

Western Blot: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4. Lane 1: CSTF3 transfected lysate(11.44 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of monoclonal antibody to CSTF3 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Western Blot: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi ( Cat # H00001479-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody (M01), clone 1D4 (Cat # H00001479-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: CSTF3 Antibody (1D4) [H00001479-M01] - Detection limit for recombinant GST tagged CSTF3 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, KD
• Clone: 1D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CSTF3 Antibody (1D4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CSTF3 (AAH09792, 1 a.a. ~ 103 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGDGATEQAAEYVPEKVKKAEKKLEENPYDLDAWSILIREAQNQPIDKARKTYERLVAQFPSSGRFWKLYIEAEVTILFYFFLYQYCSIHCSDRKQVRNIAN
• Localization: Nuclear
• Specificity: CSTF3 - cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSTF3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein and transfected lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CSTF3 Antibody (1D4) - Azide and BSA Free

• CF-1 77 kDa subunit
• Cleavage stimulation factor 77 kDa subunit
• cleavage stimulation factor subunit 3
• cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kD
• cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa
• CSTF 77 kDa subunit
• CSTF-77
• MGC117398
• MGC43001
• MGC75122

Background

The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CSTF3 Antibody (H00001479-M01)(1)

Publication using H00001479-M01

• Shankarling GS, MacDonald CC. Polyadenylation site-specific differences in the activity of the neuronal betaCstF-64 protein in PC-12 cells. Gene. 2013-10-25 [PMID: 23948079]

Bioinformatics

• Gene Symbol: CSTF3
• Entrez:
  • 1479(Human)
• OMIM:
  • 600367(Human)
• Uniprot:
  • AAH09792(Human)